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Center for Advanced Microscopy

The Center for Advanced Microscopy (formerly known as the Cell Imaging Facility) is located on the second floors of Morton and Searle Buildings at Northwestern University Feinberg School of Medicine, in downtown Chicago.

CAM offers a variety of light and electron microscopy services, and is home to the Nikon Imaging Center.

CAM  is proud to receive the Northwestern University Office for Research Outstanding Core Facility Award for: 2010, 2011, 2012, 2013, and 2014.

CAM is generously supported by Northwestern University Office of Research, Feinberg School of Medicine, Robert H. Lurie Comprehensive Cancer Center, and the NU Department of Cell & Molecular Biology.

Information for New/Prospective Users

This website provide informative guide to our portfolio of more than 30 services. Users are encouraged to discuss their project design and experimental needs with the facility staff prior to using the facility.

If you are interested in being trained on an imaging platform please contact CAM staff.  Include what type of sample you are imaging (cells, tissue, gels etc), your fluorophores, whether your sample is live or fixed and what you are hoping to visualize. Training sessions are Monday-Friday 9-12 or 1-4, and based on the availability of the staff and the instrument.  Cost for the training session is $27/hour in addition to the instrument time.  We can train up to 3 individuals at a time for light microscopy (1 at time for EM training). To schedule a training we will need a chartstring to bill you for the charges, and your NetID. We will also need your full name (as displayed on your ID),  employee ID number (located on the front of your wildcard) and the numbers on the back of your wildcard to grant you access to the facility which is locked 24/7.

New users who need to utilize laser-based instruments (confocals and laser microdissection system) are required to take the ISIS laser safety course by the State of Illinois. Please follow the links for the laser safety course and then register for the all lasers within the facility.

 

Data Storage Policy

The Center for Advanced Microscopy maintains a 10TB server for data transfer and analysis. This is for TEMPORARY transfer of data. Users are welcome to leave data that they are processing and analyzing on the server for up to 3 months.  We do not have the capacity for long term/permanent storage of user data, and thus routinely delete data older than 3 months without warning.  Please also be aware that the Terastation is NOT backed up, users are responsible for their data.

 

Acknowledging the Center for Advanced Microscopy

It is important that the use of the Center for Advanced Microscopy is properly acknowledged in your publications. Acknowledgement helps us demonstrate the impact we contribute to the research community, and it helps tremendously in our efforts to secure funding to bring you more cutting edge instruments and services.

So help us help you!

Anyone who uses the facility should acknowledge the Center for Advanced Microscopy by including a “General Acknowledgment”. If any specific equipment was used, they should ADD the corresponding paragraph that matches the instrument.

General acknowledgment

“Imaging work was performed at the Northwestern University Center for Advanced Microscopy generously supported by NCI CCSG P30 CA060553 awarded to the Robert H Lurie Comprehensive Cancer Center.”

Andor Spinning Disk

“Spinning disk confocal microscopy was performed on an Andor XDI Revolution microscope, purchased through the support of NCRR 1S10 RR031680-01.”

Nikon Multiphoton

“Multiphoton microscopy was perform on a Nikon A1R multiphoton microscope, acquired through the support of NIH 1S10OD010398-01.”

Nikon N-SIM

“Structured illumination microscopy was performed on a Nikon N-SIM system, purchased through the support of NIH 1S10OD016342-01.”

Zeiss PALM

“Microdissection was performed on Zeiss PALM laser catapulting microdissection system purchased with the support of NCRR 1S10RR025624-01.”

For example, if a user uses the PALM system, then the acknowledgment should read:

“Imaging work was performed at the Northwestern University Center for Advanced Microscopy generously supported by CCSG P30 CA060553 awarded to the Robert H Lurie Comprehensive Cancer Center. Microdissection was performed on Zeiss PALM microdissection system purchased with the support of NCRR 1S10RR025624-01.”

Users should also send a PDF copy of the papers they publish to Joshua Rappoport.

 

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