Fluorescence Microscopy

Fluorescence microscopy refers to any microscopy technique that employs excitation of a fluorophore which then emits light.  Generally speaking fluorophores are added to samples and excited at particular wavelengths and then emit at other wavelengths.  The wavelength for excitation is generally lower than that of emission, thus we use blue light to excite a green fluorophore (e.g. Green Fluorescent Protein, GFP).