Instruments

Light Microscopy

  • CRI Nuance

    •The Nuance is a spectral camera on a Zeiss Axioskop upright microscope capable of  both brightfield and fluorescence (blue, green, red) capture. It is effective at distinguishing cells stained with DAB and H&E as well as fluorescent vs autofluorescent signals.
    •The Nuance was purchased through funding granted by the Avon Foundation to Charles Clevenger.
     
  • IVIS Spectrum Bioluminescence/Fluorescence Imaging System

    •The IVIS Spectrum is capable of both in vivo bioluminescent as well as fluorescent imaging of small animals.  Bioluminescent in vivo imaging is an exceptionally sensitive method of tracking cell migration and growth in vivo.  Fluorescence animal  imaging is less sensitive, but allows multiple channel acquisition (wavelength range is 430-850nm) and supports spectral unmixing.  The IVIS spectrum allows imaging of up to 5 mice at time and can obtain a resolution of 20 microns.  There is also an optional 3D tomographic reconstruction option for more precise localization of signal in 3D.  It is housed in the pathogen free barrier in the Lurie Vivarium and accessible only by users with approved IACUC animal protocols.  Users are encouraged to consult with CAM staff  when designing their  experiments.

    •This instrument is generously provided by the Robert H. Lurie Comprehensive Cancer Center. 

     

    Instrument Status

  • LaVision UltraMicroscope II Light Sheet

    •The LaVision light sheet UltraMicroscopes allow  users to image cleared tissue in either aqueous buffers or organic solven.  It features an optical zoom from 1.26x to 12.6x.  It has a sample uptake of to 30 mm x 30 mm x 15 mm, and can image a field of view with a diagonal of 17.6mm.  There are 6 light sheets for homogenous illumination.  It has three available laser lines: 488, 561, 637.
  • Nikon AZ100 Multi-purpose Zoom Microscope

    •The AZ-100 covers a wide range of magnifications from 5X to 400X. It combines the advantages of stereo zoom microscopes and compound microscopes. The system can image blue, green and red-emitting fluorophores in thick tissues (e.g. Zebrafish, chicken eggs).  The facility has obtained funding which will allow us to convert the system into a scanning confocal macroscope upon request.  Please contact Josh or Dina for further details.
    •The AZ-100 was acquired through the support of Northwestern University Office for Research.
     
  • Nikon A1 Confocal Laser Microscope System

    •The A1 is a laser scanning confocal with two standard PMTs (408, and 640)  and two high sensitivity GaAsP detectors (488, 561) as well as multispectral detectors for accurate emission separation. The system is equipped with the PerfectFocus focal drift compensation mechanism, and automated XY stage.
    •The system was acquired through the generous support of Northwestern University Office for Research and Feinberg School of Medicine (FSM) Dean’s Office.  Additionally, several components of the system are on consignment from Nikon Instruments.

     

  • Nikon A1R+ Confocal Laser Microscope Systems (2 systems)

    •The A1R is a laser scanning confocal with resonant scanning technology can reach 400 fps raster scan speed. We currently have two instruments available.  The first system has two standard PMTs (408, and 638)  and two high sensitivity GaAsP detectors (488, 561) as well as multispectral detectors for accurate emission separation. Available laser lines are (408,458,488,515,561,638).  The second system has two standard PMTs (408, and 638)  and two high sensitivity GaAsP detectors (488, 561) for live cell or high sensitivity applications. Available laser lines are (408, 488, 561 and 638). Both systems are equipped with the PerfectFocus focal drift compensation mechanism, automated XY stage, and an incubated Tokai Hit chamber the A1R also serves as a powerful platform for multipoint live cell imaging.
    •The first system was acquired through the generous support of Northwestern University Office for Research and Feinberg School of Medicine (FSM) Dean’s Office.  The second system was purchased through the support of the Robert H. Lurie Comprehensive Cancer Center (RHLCCC) and the FSM Dean’s Office.

     

  • Nikon A1RMP+ Multiphoton

    •The Nikon A1R-MP is an upright mulitphoton microscope tailored for deep-tissue imaging with penetration depth of up to 1 – 1.5mm, depending on the sample type. The tunable Chameleon Vision titanium sapphire laser can tuned within the range of 690-1040nm for excitation of most commonly used dyes within the blue and red emission range. In addition, the system is equipped with resonant scanner, capable of scanning up to ~400 frames per second, thus making this an ideal system to perform intravital imaging, even on rapid biological processes.
    •The A1R-MP+ multiphoton microscope was acquired through an S10 shared instrumentation grant awarded to Teng- Leong Chew (1 S10 OD010398-01).
     
  • Nikon Biostation (2 systems)

    •The Nikon Biostation is an integrated cell incubator and microscopy system that allows users to conduct long-term, multi-point live cell imaging. The Biostation facilitates a broad array of time-lapse experiments by providing environmental control of temperature, humidity and gas concentration with phase and fluorescence imaging (red and green). The first system holds 35 mm glass bottom dishes and the second system is configured with a holder for chambered coverglass.
    •The first system was acquired through the generous support from Northwestern University Office for Research and Skin Disease Research Center. The seoond system was purchased through the support of the Northwestern University Office for Research.
  • Nikon N-SIM Structured Illumination Super-resolution Microscope

    •The Nikon Structured Illumination Super-resolution Microscope realizes resolution of up to 115 nm in multiple colors (405, 488, 561, 640). We are able to capture imaging in 2D,3D (with Piezo stepping) and TIRF mode, in both fixed and in some live cells. Unlike conventional confocal microscopes, which are deblurring  instruments that remove out-of-focus light through the use of pinholes, the N-SIM system utilizes moiré fringes to illuminate biological structures with spatially structured excitation light to achieve resolution beyond Ernst Abbe’s diffraction limit. Now equipped with a Tokai Hit incubated chamber, the N-SIM system is capable of live cell imaging at 37° and soon will be capable of maintaining 5% CO2.
    •Purchased through a shared instrumentation grant awarded to Teng-Leong Chew by the NIH (1S10OD016342-01).
     
  • Nikon Spinning Disk Confocal/ Nikon STochastic Optical Reconstruction Microscope

    •This inverted microscope supports widefield, TIRF, STORM and Yokogawa CSU-X1 spinning disk confocal imaging in combination with a Bruker Miniscanner capable of user-defined photobleaching and photoconversion experiments. Long-term live cell imaging experiment is supported by an Tokai stage CO2 incubator and Nikon Perfect Focus Ti microscope.  Available laser lines include (405, 445, 488, 514, 561, and 647).
    •N-STORM is able to achieve the highest resolution of all light microscopy systems within the Center for Advanced Microscopy. Storm takes advantage of the blinking properties of fluorescent molecules.  At any given point in time only a subset of molecules will be emitting light.  A series of images are captured, allowing the signal from single fluorophores to be precisely located and reconstructed into a single image. A lateral resolution of approximately 20nm, and axial of 50nm can be achieved. Please contact the staff at the Center for Advanced Microscopy to discuss which fluorophores are compatible with this imaging modality.
    •The system was acquired through a NCRR shared instrumentation grant awarded to Vladimir Gelfand (S10 RR031680-01), in conjunction with funds from the Northwestern University Office for Research, the RHLCCC and FSM Dean’s Office.  Additionally, several components of the system are on consignment from Nikon Instruments.
  • Olympus DSU and Eppendorf Femtojet Microinjector

    •The Olympus DSU is a unique spinning disk microscope which comes equipped with 3 disks of varying slit width that giving users the capability to swap disks so that they are matched to magnification. The DSU uses arc lamp illumination for excitation wavelengths in the range of 350 -700nm, allowing for imaging of reporters such as Fura dyes.  The DSU also has a Tokai Hit chamber for live cell imaging as well as a Femtojet Microinjector which injects small to intermediate volumes (up to 100pl) of aqueous solutions into cells.  (The Femtojet was generously donated by Sui Huang after being purchased by funding obtained by the Whitaker Fund.)
    •The Olympus DSU was acquired through the support of Northwestern University Office for Research.
     
  • TissueGnostics

    •TissueGnostics is a high-throughput, automated, slide imaging system.  It is capable of capturing large format, high resolution, stitched widefield images in either brightfield or 4 color fluorescence from up to 8 slides at a time. In combination with the user friendly HistoFaxs software package, researchers are able to quantify subpopulations of cells (ie double positive vs total cells) and have the areas where these cells are present be highlighted in the images.
    •The TissueGnostics system was acquired through the support of Northwestern University Office for Research.
     
  • Zeiss PALM Laser Capture Microdissection System

    •The PALM system  uses a unique combination of a high precision microdissection laser and a catapulting pulse of defocused laser to effectively transport the specimen of the obejct plane into a collection device.  The pressure catapulting mechanism eliminates heating of thermoplastic film.  Specimen are usually mounted on a poly ethylene naphthalate (PEN) or polyethylene tetraphthalate (PET) membrane.  This system is also able to dissect tissue mounted glass slides coated with a PEN membrane.  It is capable of cutting sections up to 200um thick with 2-3um precision.  This allows for live cell imaging and microdissection of subcellular structures. 
     •The Zeiss PALM microdissection system is purchased through a National Center for Research Resources (NCRR) S10 Shared Instrumentation Grant 1S10RR025624-01 awarded to Teng-Leong Chew with cost sharing from Feinberg School of Medicine.
     

Electron Microscopy

  • FEI Tecnai Spirit G2

    •The FEI Tecnai Spirit G2 is a 120kV transmission electron microscope (TEM). In addition to its greatly improved capability to generate high contrast and to image much thicker sample, the system is capable of performing 3D TEM tomography. This is a tomography technique for obtaining detailed 3D structures of subcellular macromolecular object. Using a tiltable sample holder, the sample is rotated, usually at 1 degree increment during the 3D image acquisition process, as an electron beam is therefore passed through the sample. The information is then collected and used to reconstruct a three representation image of the target. Current resolutions of 3D tomography are in the 5-20 nm range, suitable for examining supra-molecular multi-protein structures, although not the secondary and tertiary structure of an individual protein or polypeptide.  
     
    •It was purchased with the support of Northwestern University Office for Research.  Please contact Lennell for more details or training.
     
  • JEOL NeoScope SEM

    •The NeoScope benchtop SEM complements both optical microscopes and traditional SEMs in the lab, and can be configured for advanced analytical applications. This compact electron microscope has the powerful electron optics of an SEM, with up to 60,000X magnification and offers both an Everhart Thornley type SE detector as well as high sensitivity solid state BSE detector. The microscope has a sleek new design with up-to-date features. Operation is via a touch screen, and is simplified with auto focus, auto alignment, auto contrast and auto brightness controls. The NeoScope operates in both low and high vacuum modes with three settings for accelerating voltage. These parameters are suitable for a variety of applications, all of which can be programmed in special pre-stored recipe files.

    •The system was acquired through the support of the Office for Research with cost share from the FSM Dean’s Office.

  • Leica Freeze Substitution System

    •This unit can be programmed to perform stepwise, microprocessor-controlled changes in fixation conditions for user samples.  It was purchased through the support of the Office for Research with cost share from RHLCCC.
     

     

  • Leica Microtome UCT

    •The Leica Ultracut UCT uses either a diamond or glass knife to cut ultra thin sections of specimens. It’s ideal for biological application and soft materials preparation. Features; automatic feed in 1nm to 100 nm. Cutting speed can be controlled to .05 mm per second. It allows longitudinal and cross sectioning. Key functions can be operated from the separate control unit.  Please contact Lennell for more details.

     Instrument Status


  • LKB Knifemaker

    This knife maker allows users to create glass knives for electron and light microscopy sectioning.  The thickness of glass used is 6-7mm. Please contact Lennell Reynolds for additional information.

    Instrument Status