Epi-fluorescence microscopy

Often referred to as “widefield” microscopy, epi-fluorescence employs illumination of the entire sample field in XY and Z and is generally recorded through use of a camera (e.g. a CCD).  Although relative cheap and easy to employ, epi-fluorescence microscopy suffers from contamination of out-of-focus light in the imaging plane.  Techniques such as deconvolution, confocal microscopy or TIRF microscopy can be employed for samples for which out-of-focus light precludes the ability to successfully perform an experiment.